Panel Builder
by Anthony Desbien, PhD
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Inputs
Species
Mouse
Human
Rat
Non-human primate
Other
If other, specify
Tissue / sample
Lung
Blood / PBMC
Spleen
Tumor (TME)
Lymph node
BAL
Bone marrow
Liver
Other
If other, specify
Research goal
Major immune subsets
High-res myeloid
T cell activation / exhaustion
Innate / NK / ILC
Infection / inflammation
Immuno-oncology (TME)
Other
If other, specify
Disease state
(optional)
Cytometer / max colors
BD LSRFortessa (4-laser)
Cytek Aurora (spectral)
Sony MA900 (5-laser)
BD FACSymphony A5
Other
Must-include markers
(optional, one per line)
Constraints / notes
(optional)
- Use NIR Live/Dead (viability dye) - CD45 on BB515 - Avoid PerCP-Cy5.5
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Markers & Fluors
Populations
Marker
Priority
Clone
Fluorochrome
Antigen density
Rationale